填充柱超临界流体色谱法分析结合胆汁酸

Analysis of conjugated bile acids by packed-column supercritical fluid chromatography.

作者信息

Scalia S, Games D E

机构信息

Department of Chemistry, University College of Swansea, Singleton Park, UK.

出版信息

J Chromatogr. 1992 Feb 14;574(2):197-203. doi: 10.1016/0378-4347(92)80030-t.

DOI:10.1016/0378-4347(92)80030-t

PMID:1618950

Abstract

A rapid method has been developed for the simultaneous separation of the polar glycine- and taurine-conjugated bile acids by packed-column supercritical fluid chromatography. Samples were analysed on a cyanopropyl-bonded silica column with ultraviolet detection at 210 nm and carbon dioxide modified with methanol as the mobile phase. The influence of the stationary phase, modifier concentration, temperature, column pressure and modifier identity on retention was also studied. This new chromatographic method is applicable to the assay of conjugated bile acids in duodenal bile samples from patients with hepatobiliary diseases.

摘要

已开发出一种快速方法，用于通过填充柱超临界流体色谱法同时分离极性甘氨酸和牛磺酸共轭胆汁酸。样品在氰丙基键合硅胶柱上进行分析，采用210 nm紫外检测，以甲醇改性的二氧化碳为流动相。还研究了固定相、改性剂浓度、温度、柱压和改性剂种类对保留的影响。这种新的色谱方法适用于测定肝胆疾病患者十二指肠胆汁样品中的共轭胆汁酸。