Del Cont Bernard A, Royer Morrot M J, Zhiri A, Rambourg M, Royer R J
Département de Pharmacologie Clinique, CHRU, Nancy, France.
J Chromatogr. 1992 Feb 14;574(2):365-8. doi: 10.1016/0378-4347(92)80055-u.
A rapid and simple column liquid chromatographic method involving a column switching system for the determination of disopyramide and its N-monodealkyl metabolite (NMD) in plasma is described. The deproteinized plasma is applied to an automated system. Purification and concentration were performed using a precolumn connected to a six-position valve; analytical separation was done on-line using a cyano reversed-phase column with a mobile phase consisting of 10 mmol/l trimethylamine (pH 2.5, adjusted with phosphoric acid)-acetonitrile-tetrahydrofuran (78:20:2, v/v/v). Absorbance was measured at 265 nm, with a minimum detectable amount of disopyramide and NMD of 0.1 micrograms/ml. The method can be applied to drug monitoring and pharmacokinetic studies.
描述了一种快速简便的柱液相色谱法，该方法涉及一个柱切换系统，用于测定血浆中的丙吡胺及其N-单脱烷基代谢物（NMD）。将去蛋白血浆应用于自动化系统。使用连接到六位阀的预柱进行纯化和浓缩；使用氰基反相柱进行在线分析分离，流动相由10 mmol/l三甲胺（用磷酸调节pH至2.5）-乙腈-四氢呋喃（78:20:2，v/v/v）组成。在265 nm处测量吸光度，丙吡胺和NMD的最低检测量为0.1微克/毫升。该方法可用于药物监测和药代动力学研究。
