Swezey C B, Ponzo J L
Ther Drug Monit. 1984;6(2):211-4. doi: 10.1097/00007691-198406000-00014.
A method is described for the quantitation of disopyramide phosphate (Norpace) in serum by high-performance liquid chromatography. The analysis utilizes ultraviolet detection at 254 nm with a 5-micrometers reversed-phase column and a mobile phase consisting of acetonitrile, ammonium dihydrogen phosphate buffer, acetic acid, and water. The determination requires 100 microliters of serum sample and the analysis time (9 min) is rapid. Serum extraction is a two-step process using chloroform, sodium carbonate, and hydrochloric acid. Although acid is used, columns produced sharp peaks with consistent retention times for 6-8 months. This method provides selectivity and sensitivity with a precision of 3.8%, average recovery of 92.9% and no interference from 18 commonly administered drugs.
描述了一种通过高效液相色谱法定量测定血清中磷酸丙吡胺（诺帕斯）的方法。该分析采用在254nm处的紫外检测，使用5微米反相柱和由乙腈、磷酸二氢铵缓冲液、乙酸和水组成的流动相。该测定需要100微升血清样品，分析时间（9分钟）很快。血清提取是一个使用氯仿、碳酸钠和盐酸的两步过程。虽然使用了酸，但色谱柱在6 - 8个月内产生了尖锐的峰，保留时间一致。该方法具有选择性和灵敏度，精密度为3.8%，平均回收率为92.9%，且不受18种常用药物的干扰。
