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采用高效液相色谱法以及带有化学电离的毛细管气相色谱 - 质谱联用法对藻类和酵母中的甾醇酯进行分析。

Analysis of sterol esters from alga and yeast by high-performance liquid chromatography and capillary gas chromatography-mass spectrometry with chemical ionization.

作者信息

Rezanka T

机构信息

Institute of Microbiology, Prague, Czechoslovakia.

出版信息

J Chromatogr. 1992 May 15;598(2):219-26. doi: 10.1016/0021-9673(92)85051-t.

DOI:10.1016/0021-9673(92)85051-t
PMID:1618981
Abstract

Sterol esters from the green alga Chlorella kessleri and the yeast Saccharomyces cerevisiae were analysed by tandem high-performance liquid chromatography (HPLC-HPLC). Non-polar lipids were separated by normal-phase HPLC into individual classes and the fraction of sterol esters was subsequently separated by reversed-phase HPLC into intact molecular species. Further separation and identification of the fractions after HPLC were effected by capillary gas chromatography-mass spectrometry (cGC-MS) with positive and/or negative chemical ionization. A technique consisting of direct injection of the sample after its passage through the detector into the on-column injector of the gas chromatograph was used to transfer the sample from HPLC-HPLC to cGC-MS. By means of this method it was possible to demonstrate more than 30 sterols in both the alga and the yeast, and more than 20 new sterol esters were detected.

摘要

采用串联高效液相色谱法（HPLC-HPLC）对来自小球藻和酿酒酵母的甾醇酯进行了分析。通过正相HPLC将非极性脂质分离成各个类别，随后通过反相HPLC将甾醇酯部分分离成完整的分子种类。HPLC之后各部分的进一步分离和鉴定通过带有正化学电离和/或负化学电离的毛细管气相色谱-质谱联用仪（cGC-MS）进行。使用一种在样品通过检测器后直接注入气相色谱仪柱上进样器的技术，将样品从HPLC-HPLC转移至cGC-MS。通过该方法，在藻类和酵母中均检测到30多种甾醇，并且检测到20多种新的甾醇酯。

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