腺病毒介导的基因转移在嗅上皮和嗅球中的长期研究。
Adenovirus-mediated gene transfer in olfactory epithelium and olfactory bulb: a long-term study.
作者信息
Doi Kiyoshi, Nibu Ken-ichi, Ishida Haruhiko, Okado Haruo, Terashima Toshio
机构信息
Dept of Otolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho 7-5-1, Chuo-Ku, Kobe 650-0017, Japan.
出版信息
Ann Otol Rhinol Laryngol. 2005 Aug;114(8):629-33. doi: 10.1177/000348940511400808.
OBJECTIVES
We sought to study the spatiotemporal gene expression mediated by adenoviral vector in the olfactory pathways.
METHODS
The replication-defective adenoviral vector AxCALacZ, which encodes the enzyme Escherichia coli beta-galactosidase, was applied to mouse olfactory epithelium by intranasal instillation.
RESULTS
The LacZ gene product, beta-galactosidase, was expressed not only in the olfactory receptor neurons and their axons, but also in the olfactory bulbs. The first evidence of anterograde labeling was observed at postinfection day (PID) 2. At PID 3, beta-galactosidase was strongly expressed in olfactory nerve axons, as well as their terminal glomeruli, in the olfactory bulbs. beta-Galactosidase expression persisted up to PID 90, and there was a significant decrease in the number of labeled neurons at PID 30.
CONCLUSIONS
These results suggest possible long-term effects of adenovirus-mediated gene transfer on the olfactory neurons, as well as the olfactory bulbs.
目的
我们试图研究腺病毒载体在嗅觉通路中介导的时空基因表达。
方法
将编码大肠杆菌β-半乳糖苷酶的复制缺陷型腺病毒载体AxCALacZ通过滴鼻法应用于小鼠嗅觉上皮。
结果
LacZ基因产物β-半乳糖苷酶不仅在嗅觉受体神经元及其轴突中表达，也在嗅球中表达。在感染后第2天观察到顺行标记的首个证据。在感染后第3天，β-半乳糖苷酶在嗅神经轴突及其在嗅球中的终末小球中强烈表达。β-半乳糖苷酶表达持续至感染后第90天，且在感染后第30天标记神经元数量显著减少。
结论
这些结果提示腺病毒介导的基因转移对嗅觉神经元以及嗅球可能具有长期影响。
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