Yan Li, Han Ying, He Yuanlong, Xie Huahong, Liu Jingmei, Zhao Lina, Wang Jingbo, Gao Liuchun, Fan Daiming
Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, 17 Changle Western Road, Xi'an, Shaanxi Province, 710032, China.
Stem Cell Rev. 2007 Dec;3(4):265-9. doi: 10.1007/s12015-007-9004-y.
Pluripotent stem cells have shown great therapeutic promise because of their natural capacity to regenerate damaged tissue. Likewise, autologous stem cells or genetically modified stem cells have already been successfully applied in animal or clinical experimental studies including cardiopathy, diabetic disease, system lupus erythema, pancreatic disease, and liver disease. In these studies regarding stem cell transplants in different diseases, identifying the location of implanted cells and distinguishing them from endogenous cells is the first and most important step. Moreover, different tracing techniques were applied in different studies for their different sensitivity, dynamic range, convenience and reliability of their assays. Therefore, we will here review different tracing techniques and their applications in stem cell transplants, including both experiment studies and preclinical trials.
多能干细胞因其具有再生受损组织的天然能力而展现出巨大的治疗前景。同样，自体干细胞或基因改造干细胞已成功应用于动物或临床实验研究，包括心脏病、糖尿病、系统性红斑狼疮、胰腺疾病和肝脏疾病。在这些关于不同疾病干细胞移植的研究中，确定植入细胞的位置并将它们与内源性细胞区分开来是首要且最重要的一步。此外，不同的示踪技术因其检测的敏感性、动态范围、便利性和可靠性不同而应用于不同的研究。因此，我们将在此综述不同的示踪技术及其在干细胞移植中的应用，包括实验研究和临床试验。
