Chua K B, Chua K H, Chua I-Ly, Chen K F
National Public Health Laboratory, Ministry of Health Malaysia, Malaysia.
Malays J Pathol. 2004 Jun;26(1):69-71.
Virus isolation and accurate characterization plays a crucial role in the rapid identification of the causative agents of infectious disease outbreaks especially if the causative viruses are novel where no pre-existing diagnostic reagents would be available. A new cell culture tube, named Jui Meng (JM) Cell Culture Tube, was developed to reduce the cost and improve the efficiency and biosafety of work pertaining to virus isolation. The design of the tube is based heavily on the principle of practicability, functionality, biosafety and long-term cost saving for diagnostic laboratory work in virus isolation. It is designed to culture an initial inoculum of one milliliter of culture medium containing 1 x 10(4) to 1 x 10(5) cells/ml.
病毒分离和准确鉴定在快速识别传染病暴发的病原体方面起着至关重要的作用,尤其是在病原体为新型病毒且没有现成诊断试剂的情况下。一种名为瑞萌(JM)细胞培养管的新型细胞培养管被研发出来,以降低成本并提高病毒分离相关工作的效率和生物安全性。该培养管的设计主要基于实用性、功能性、生物安全性以及为病毒分离诊断实验室工作长期节省成本的原则。它被设计用于培养初始接种量为一毫升、含有每毫升1×10⁴至1×10⁵个细胞的培养基。