Nkya W M, Warren R Q, Wolf H, Hendrix C W, Tesha J, Redfield R R, Melcher G P, Burke D S, Kanda P, Kennedy R C
Kilimanjaro Christian Medical Centre, Moshi, Tanzania.
J Med Virol. 1992 May;37(1):61-6. doi: 10.1002/jmv.1890370111.
A total of 160 sera from HIV-1 infected individuals from Tanzania were examined for their fine specificity characteristics relative to 9 synthetic peptides that define HIV-1 gp160 epitopes. Immunorecessive and immunodominant epitopes were identified in both gp120 and gp41 based on serologic reactivity of these HIV-1 infected sera. A significant difference in fine specificity among HIV-1 infected individuals from Tanzania and the United States was observed for an immunodominant gp41 epitope. No significant differences in reactivity among asymptomatic vs. symptomatic HIV-1 infected individuals were detected for the selected HIV-1 gp160 epitopes defined by these peptides. The majority of sera from HIV-1 infected Tanzanians contained antibodies that recognized a peptide corresponding to the V3 region of gp120 from the HIV-1 MN isolate. These data suggest that regional isolates of HIV-1 may exist in Tanzania that differ from HIV-1 isolated in the United States. However, based on serology, HIV-1 isolates exhibiting sequences with HIV-1 MN V3 similarity may also be prevalent in Tanzania. The results of this study may be useful for the design of more effective AIDS diagnostic and therapeutic products for use worldwide.
对来自坦桑尼亚的160份HIV-1感染者血清进行检测，以研究其针对9种界定HIV-1 gp160表位的合成肽的精细特异性特征。根据这些HIV-1感染者血清的血清学反应性，在gp120和gp41中均鉴定出免疫隐性和免疫显性表位。对于一个免疫显性的gp41表位，观察到坦桑尼亚和美国的HIV-1感染者在精细特异性上存在显著差异。对于这些肽所界定的选定HIV-1 gp160表位，无症状与有症状的HIV-1感染者之间未检测到反应性的显著差异。大多数来自HIV-1感染的坦桑尼亚人的血清含有能识别与HIV-1 MN分离株gp120的V3区相对应肽段的抗体。这些数据表明，坦桑尼亚可能存在与美国分离的HIV-1不同的HIV-1区域分离株。然而，基于血清学，具有与HIV-1 MN V3相似序列的HIV-1分离株在坦桑尼亚也可能很普遍。本研究结果可能有助于设计更有效的用于全球的艾滋病诊断和治疗产品。
