Warren R Q, Nkya W M, Shao J F, Anderson S A, Wolf H, Hendrix C W, Kanda P, Wabuke M, Boswell R N, Redfield R R
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78284.
J Clin Microbiol. 1992 Jan;30(1):126-31. doi: 10.1128/jcm.30.1.126-131.1992.
In this study, we compared sera from 159 human immunodeficiency virus type 1 (HIV-1)-infected individuals from Tanzania and 103 infected individuals from the United States for antibodies reactive with 10 HIV-1 gp160 epitopes defined by synthetic peptides. Our data indicate that the anti-gp160 antibody fine specificity differs between infected individuals from these two geographically diverse populations. For example, 50% of the Tanzanian sera contained antibodies reactive with an immunodominant HIV-1 gp41 epitope defined by peptide 600-611, whereas 91% of the sera from the United States were reactive. Differences in serologic reactivity between HIV-1-infected individuals from Tanzania and the United States were also observed with gp160 epitopes defined by peptides 503-528 and 846-860. Included among the peptides examined were four which corresponded to the V3 region of gp120. The majority of sera from either country contained antibodies reactive with peptide RP142, whose V3 sequence is based upon that of HIV-1 isolate MN. Further characterization of serologic reactivity suggested that sera from Tanzania were more likely to neutralize HIV-1 isolate IIIB or MN in vitro than were sera from the United States. These differences in antibody fine specificity between HIV-1-infected individuals from Tanzania and the United States suggest that regional isolates of HIV-1 may exist.
在本研究中，我们比较了来自坦桑尼亚的159名1型人类免疫缺陷病毒（HIV-1）感染者和来自美国的103名感染者的血清，检测其与10种由合成肽定义的HIV-1 gp160表位发生反应的抗体。我们的数据表明，这两个地理上不同人群的感染者中，抗gp160抗体的精细特异性存在差异。例如，50%的坦桑尼亚血清含有与由肽600 - 611定义的免疫显性HIV-1 gp41表位发生反应的抗体，而来自美国的血清中有91%呈反应性。在由肽503 - 528和846 - 860定义的gp160表位上，也观察到了坦桑尼亚和美国的HIV-1感染者之间血清反应性的差异。所检测的肽中包括四种与gp120的V3区域相对应的肽。来自这两个国家的大多数血清都含有与肽RP142发生反应的抗体，其V3序列基于HIV-1分离株MN。血清反应性的进一步特征表明，坦桑尼亚的血清在体外比美国的血清更有可能中和HIV-1分离株IIIB或MN。坦桑尼亚和美国的HIV-1感染者之间抗体精细特异性的这些差异表明，可能存在HIV-1的区域分离株。
