Wilson Jeremy D, Foster Thomas H
Department of Radiology, University of Rochester, 601 Elmwood Avenue, Box 648, Rochester, New York 14642, USA.
Opt Lett. 2005 Sep 15;30(18):2442-4. doi: 10.1364/ol.30.002442.
We present a Mie theory-based analysis of angularly resolved light scattering from intact cells that involves the product of a particle size distribution rho and the scattering cross section sigma. The analysis shows that the ability to size intracellular organelles depends strongly upon the assumed functional form of the particle size distribution; however, the product sigma rho extracts the dominant light scatterers regardless of this choice. We examine goniometer measurements from our own and another laboratory and find that this analysis reconciles seemingly conflicting results and confirms that mitochondrial-sized organelles dominate scattering from intact cells in the angular range 50 degrees - 90 degrees.
我们提出了一种基于米氏理论的对完整细胞角分辨光散射的分析方法，该方法涉及粒径分布ρ与散射截面σ的乘积。分析表明，对细胞内细胞器进行尺寸测定的能力在很大程度上取决于所假定的粒径分布函数形式；然而，无论做出何种选择，乘积σρ都能提取出主要的光散射体。我们研究了来自我们自己实验室和另一个实验室的测角仪测量结果，发现这种分析方法调和了看似相互矛盾的结果，并证实了线粒体大小的细胞器在50度至90度的角度范围内主导了完整细胞的散射。
