Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, 96 Jinzhai Road, Hefei 230027, China.
Sensors (Basel). 2017 Jul 7;17(7):1592. doi: 10.3390/s17071592.
Raman spectroscopy is a label-free method of obtaining detailed chemical information about samples. Its compatibility with living tissue makes it an attractive choice for biomedical analysis, yet its translation from a research tool to a clinical tool has been slow, hampered by fundamental Raman scattering issues such as long integration times and limited penetration depth. In this review we detail the how combining Raman spectroscopy with other techniques yields multimodal instruments that can help to surmount the translational barriers faced by Raman alone. We review Raman combined with several optical and non-optical methods, including fluorescence, elastic scattering, OCT, phase imaging, and mass spectrometry. In each section we highlight the power of each combination along with a brief history and presentation of representative results. Finally, we conclude with a perspective detailing both benefits and challenges for multimodal Raman measurements, and give thoughts on future directions in the field.
拉曼光谱是一种获取样品详细化学信息的无标记方法。由于其与活体组织的兼容性，使其成为生物医学分析的理想选择，但它从研究工具到临床工具的转化一直很缓慢，这是由于拉曼散射的一些基本问题，例如长积分时间和有限的穿透深度。在这篇综述中，我们详细介绍了如何将拉曼光谱与其他技术相结合，产生多模态仪器，以帮助克服拉曼技术本身所面临的转化障碍。我们综述了拉曼与几种光学和非光学方法的结合，包括荧光、弹性散射、OCT、相衬成像和质谱。在每个部分中，我们都强调了每种组合的优势，以及简要的历史和代表性结果的介绍。最后，我们从细节上阐述了多模态拉曼测量的优势和挑战，并对该领域的未来方向进行了思考。
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