Kar Deepayan, Kim Yeon Jin, Packer Orin, Clark Mark E, Cao Dongfeng, Owsley Cynthia, Dacey Dennis M, Curcio Christine A
Department of Ophthalmology and Visual Sciences, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Department of Biological Structure, University of Washington, Seattle, WA, USA.
Biomed Opt Express. 2023 Sep 27;14(10):5512-5527. doi: 10.1364/BOE.501228. eCollection 2023 Oct 1.
Mitochondria are candidate reflectivity signal sources in optical coherence tomography (OCT) retinal imaging. Here, we use deep-learning-assisted volume electron microscopy of human retina and imaging to map mitochondria networks in the outer plexiform layer (OPL), where photoreceptors synapse with second-order interneurons. We observed alternating layers of high and low mitochondrial abundance in the anatomical OPL and adjacent inner nuclear layer (INL). Subcellular resolution OCT imaging of human eyes revealed multiple reflective bands that matched the corresponding INL and combined OPL sublayers. Data linking specific mitochondria to defined bands in OCT may help improve clinical diagnosis and the evaluation of mitochondria-targeting therapies.
线粒体是光学相干断层扫描（OCT）视网膜成像中潜在的反射信号源。在此，我们利用深度学习辅助的人体视网膜体积电子显微镜和成像技术，绘制外网状层（OPL）中的线粒体网络，该层是光感受器与二级中间神经元形成突触的部位。我们在解剖学上的OPL和相邻的内核层（INL）中观察到线粒体丰度高低交替的层。人眼的亚细胞分辨率OCT成像显示出多个反射带，与相应的INL和合并的OPL亚层相匹配。将特定线粒体与OCT中确定的带联系起来的数据，可能有助于改善临床诊断和评估针对线粒体的治疗方法。
