Mok P L, Leong C F, Cheong S K
Clinical Haematology and Stem Cell Transplantation Services, MAKNA-HUKM Cancer Institute, Cheras, Kuala Lumpur.
Malays J Pathol. 2003 Dec;25(2):121-7.
Mesenchymal stem cells are pluripotent progenitor cells found in bone marrow that have the capacity of differentiating into bone, cartilage, tendon, fat, muscle, and early progenitors of neural cells under certain conditions. It has also been shown that they support haematopoiesis in culture. We have successfully isolated mesenchymal stem cells from a bone marrow sample of an anaemic patient by using DMEM supplemented with 10% of FBS. They could be distinguished from other cells by their tendency to adhere to tissue culture plastic. The adherent time was about three to seven days. Microscopically, the cultured cells showed morphology resembling fibroblast and they divided actively. Early passage cultures were heterogenous and contained four morphologically distinct cell types; long spindle-shaped cells, star-shaped cells, petal-shaped cells and large flattened granular cells with vacuoles. Identification of mesenchymal stem cells was carried out by cytochemical analysis and immunophenotyping by flow cytometry. Identification of these cells is vital as they have properties that appear to make them ideal candidates for studying differentiation and make them suitable for cellular and gene therapy.
间充质干细胞是存在于骨髓中的多能祖细胞，在特定条件下具有分化为骨、软骨、肌腱、脂肪、肌肉以及神经细胞早期祖细胞的能力。研究还表明，它们在培养中能支持造血作用。我们通过使用添加10%胎牛血清的DMEM培养基，成功从一名贫血患者的骨髓样本中分离出了间充质干细胞。它们可通过易于贴附于组织培养塑料的特性与其他细胞区分开来。贴附时间约为三到七天。在显微镜下，培养的细胞呈现出类似成纤维细胞的形态，并且能活跃地分裂。早期传代培养物具有异质性，包含四种形态上不同的细胞类型：长梭形细胞、星形细胞、花瓣形细胞以及带有空泡的大扁平颗粒细胞。间充质干细胞的鉴定通过细胞化学分析和流式细胞术免疫表型分析来进行。对这些细胞进行鉴定至关重要，因为它们具有的特性似乎使其成为研究分化的理想候选者，并且适合用于细胞和基因治疗。
