Noorali Samina, Nasir Muhammad Israr, Pervez Shahid
Department of Pathology and Microbiology, The Aga Khan University Hospital, Karachi, Pakistan.
J Coll Physicians Surg Pak. 2005 Jul;15(7):404-8.
To characterize angioimmunoblastic T-cell lymphoma (AILT) on morphological, immunohistochemical and molecular grounds and its association with Epstein-Barr virus (EBV) in Pakistani patients.
Case series.
Histopathology section of the Department of Pathology and Microbiology, The Aga Khan University Hospital, Karachi from January 01, 1992 to December 31, 2002.
Over a period of 11 years archival biopsy material of 13 AILT cases (lymph nodes), identified on the basis of histological and immunohistochemical criteria, using REAL and WHO classifications, were retrieved from the files of Department of Pathology. Immunophenotyping was carried out by using CD45 (LCA), two T-cell markers CD45RO (UCHL1; monoclonal) and CD3 (polyclonal). Polymerase chain reaction (PCR) was used to assess T-cell clonality for T-cell receptor (TCR)-b, g and immunoglobulin heavy chain (IgH) for FR2 and FR3 regions using primers recognizing conserved sequences of the variable (V), diversity (D) and joining (J) region segments. Association of EBV in AILT cases was studied by PCR and in situ hybridization (ISH).
This study showed AILT to constitute 0.71% of all NHLs (non-Hodgkin's lymphoma) [both T and B]. Immunohistochemical study revealed that the tumor cells were positive for CD45 (LCA), CD45RO (UCHL1) and CD3. All the 13 cases were largely negative for CD20 (L26), a B-cell marker, except few large scattered cells labelling. DNA extracted from all 13 lymph nodes was amplified using polymerase chain reaction (PCR). PCR technique demonstrated clonal gene rearrangement of the TCR-b, g and IgH regions in 3 (23.1%), 7 (53.8%) and 3 (23.1%) AILT cases, respectively out of 13 cases. Association of EBV was seen in 11 out of 13 cases (84.6%) of AILT by PCR. By ISH the prevalence of EBV was detected in 8 (88.8%) out of 9 cases.
The prevalence of AILT in the Pakistani population is slightly lower compared to other studies and that EBV is an etiological agent in pathogenesis of this disease.
从形态学、免疫组织化学和分子层面描述血管免疫母细胞性T细胞淋巴瘤（AILT）及其在巴基斯坦患者中与爱泼斯坦-巴尔病毒（EBV）的关联。
病例系列研究。
1992年1月1日至2002年12月31日期间，在卡拉奇阿迦汗大学医院病理与微生物学系的组织病理学科室进行研究。
根据组织学和免疫组织化学标准，采用REAL和世界卫生组织分类法，从病理科档案中检索出13例AILT（淋巴结）病例11年间的存档活检材料。通过使用CD45（白细胞共同抗原，LCA）、两种T细胞标志物CD45RO（UCHL1；单克隆抗体）和CD3（多克隆抗体）进行免疫表型分析。采用聚合酶链反应（PCR），使用识别可变区（V）、多样性区（D）和连接区（J）片段保守序列的引物，评估T细胞受体（TCR）-β、γ和免疫球蛋白重链（IgH）在FR2和FR3区域的T细胞克隆性。通过PCR和原位杂交（ISH）研究AILT病例中EBV的关联情况。
本研究表明AILT占所有非霍奇金淋巴瘤（NHL）（包括T细胞和B细胞淋巴瘤）的0.71%。免疫组织化学研究显示肿瘤细胞CD45（LCA）、CD45RO（UCHL1）和CD3呈阳性。13例病例中除少数散在大细胞呈阳性外，CD20（L26，一种B细胞标志物）大多呈阴性。使用聚合酶链反应（PCR）对13个淋巴结提取的DNA进行扩增。PCR技术显示，13例AILT病例中，分别有3例（23.1%）、7例（53.8%）和3例（23.1%）的TCR-β、γ和IgH区域出现克隆性基因重排。通过PCR检测，13例AILT病例中有11例（84.6%）检测到EBV关联。通过ISH检测，9例病例中有8例（88.8%）检测到EBV感染。
与其他研究相比，AILT在巴基斯坦人群中的患病率略低，且EBV是该疾病发病机制中的一种致病因素。
