Fan Yi, Zhang Min, Feng Yu-Qi
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
J Chromatogr A. 2005 Dec 16;1099(1-2):84-91. doi: 10.1016/j.chroma.2005.09.028. Epub 2005 Sep 28.
In-tube solid-phase microextraction (SPME) based on a poly(acrylamide-vinylpyridine-N,N'-methylene bisacrylamide) monolithic capillary was investigated and on-line coupled to HPLC for the determination of trace analytes in aqueous samples. The polymer monolith was conveniently synthesized in a fused silica capillary by in situ polymerization method. Several groups of analytes including non-steroidal anti-inflammatory drugs, phenols, non-peptide angiotensin II receptor antagonists and endocrine disrupting chemicals were extracted by the monolithic capillary. High extraction efficiency was achieved for the analytes investigated and great improvement of the limits of detection were obtained in comparison to that of direct chromatographic analysis and strong hydrophobic and ion-exchange interactions between the analytes and the polymer were confirmed. The newly developed monolithic capillary showed excellent reusability and high stability under extreme pH conditions during extraction. The possibility of applying the established method to water sample analysis was also demonstrated.
研究了基于聚（丙烯酰胺 - 乙烯基吡啶 - N，N'-亚甲基双丙烯酰胺）整体柱毛细管的管内固相微萃取（SPME），并将其与HPLC在线联用，用于测定水样中的痕量分析物。通过原位聚合法在熔融石英毛细管中方便地合成了聚合物整体柱。几组分析物，包括非甾体抗炎药、酚类、非肽类血管紧张素II受体拮抗剂和内分泌干扰化学物质，被整体柱毛细管萃取。所研究的分析物实现了高萃取效率，与直接色谱分析相比，检测限有了很大提高，并证实了分析物与聚合物之间存在强疏水和离子交换相互作用。新开发的整体柱毛细管在萃取过程中在极端pH条件下表现出优异的可重复使用性和高稳定性。还证明了将所建立的方法应用于水样分析的可能性。
