Ghosh Papiya, Besra Shila E, Tripathi Gayatri, Mitra Smita, Vedasiromoni Joseph R
Drug Development Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata, 700032 West Bengal, India.
Leuk Res. 2006 Apr;30(4):459-68. doi: 10.1016/j.leukres.2005.08.018. Epub 2005 Sep 28.
The anticancer activity of di- and tri-terpenes and other polyphenolic compounds present in tea is already reported. We evaluated the cytotoxic and apoptogenic effect of tea root extract (TRE) and two of its steroidal saponins named as TS1 and TS2, on human cell lines and on cells from leukemic patients. It was found that TRE, TS1 and TS2 significantly decreased cell count and that TRE caused apoptosis, as confirmed morphologically by confocal microscopy and by flow-cytometric analysis using Annexin-V FITC and propidium iodide (PI). Cell count and MTT assay in normal white blood cells (WBC) of healthy volunteers revealed that TRE produced insignificant reduction in cell count and cytotoxicity.
茶中存在的二萜和三萜以及其他多酚类化合物的抗癌活性已有报道。我们评估了茶根提取物（TRE）及其两种名为TS1和TS2的甾体皂苷对人细胞系和白血病患者细胞的细胞毒性和凋亡诱导作用。结果发现，TRE、TS1和TS2显著降低了细胞数量，并且TRE导致了细胞凋亡，这通过共聚焦显微镜以及使用膜联蛋白V FITC和碘化丙啶（PI）的流式细胞术分析在形态学上得到了证实。对健康志愿者的正常白细胞（WBC）进行细胞计数和MTT测定显示，TRE对细胞计数和细胞毒性的降低不显著。
