Spiekerman A M, Hetherly M E, Hall F F
Clin Chem. 1975 Dec;21(13):1981-2.
High-voltage paper electrophoresis of small samples of serum and urine at pH 6.0 resolves basic and acidic amino acids and separates them from the neutral amino acids. For separation and identification of the neutral amino acids, the appropriate area of the electrophoretogram is cut out, sewn onto a second sheet of paper, and rerun at pH 1.9. By this method, amino acids are rapidly resolved. It is suited for use with special procedures such as oxidation of biological fluid with performic acid and specific staining for confirmation of amino acid identification.
在pH 6.0条件下，对血清和尿液小样本进行高压纸电泳，可分离出碱性和酸性氨基酸，并将它们与中性氨基酸分开。为了分离和鉴定中性氨基酸，将电泳图谱的适当区域剪下，缝到另一张纸上，然后在pH 1.9条件下重新进行电泳。通过这种方法，氨基酸能快速得到分离。它适用于一些特殊的操作程序，如用过甲酸氧化生物流体以及进行特定染色以确认氨基酸的鉴定结果。
