Shao Z, Liu G, Ding J
Department of Surgery, Laboratory for Molecular Biology, Shanghai Cancer Hospital, Medical Center of Fudan University, Shanghai 200032, China.
Zhonghua Wai Ke Za Zhi. 2001 Oct;39(10):796-8.
To establish and characterize human inflammatory breast cancer xenograft in nude mice.
Animal studies, Northern and Western blot, zymograms and immunohistochemistry were used in our studies.
Human transplantable inflammatory carcinoma were established in nude mice. This xenograft as its human counterpart exhibited striking erythema of the overlying skin, lympho-vascular invasion and other similar biological characteristics. It also exhibited lung metastasis 4-6 weeks after growth. This xenograft was ER, PR, Her-2/neu negative and p53, EGFR positive. Comparative studies of inflammatory breast cancer xenograft with non-inflammatory xenograft indicated 10-20 fold overexpression of E-cadherin and MUC1.
This human inflammatory breast carcinoma xenograft will provide an excellent animal model to allow us to further dissect out both the upstream regulatory machinery and downstream effector molecules responsible for the inflammatory carcinoma phenotype.
在裸鼠中建立并鉴定人炎性乳腺癌异种移植模型。
本研究采用动物实验、Northern和Western印迹、酶谱分析及免疫组织化学方法。
在裸鼠中建立了人可移植性炎性癌模型。该异种移植瘤与其对应的人肿瘤一样，表现出覆盖皮肤显著红斑、淋巴管浸润及其他相似生物学特性。生长4 - 6周后还出现肺转移。该异种移植瘤雌激素受体（ER）、孕激素受体（PR）、人表皮生长因子受体2/neu（Her-2/neu）阴性，p53、表皮生长因子受体（EGFR）阳性。炎性乳腺癌异种移植瘤与非炎性异种移植瘤的比较研究表明，E-钙黏蛋白和黏蛋白1（MUC1）过表达10 - 20倍。
这人炎性乳腺癌异种移植瘤将提供一个优良的动物模型，使我们能够进一步剖析导致炎性癌表型的上游调控机制和下游效应分子。
