Pan Z, Fan Q, Wang C
Orthopaedic Institute of People's Liberation Army, 89th Hospital, Weifang 261021, China.
Zhonghua Wai Ke Za Zhi. 2001 Oct;39(10):799-801.
To investigate the biological stabilities and the identification of a human highly metastatic osteosarcoma subpopulation.
Tumor pieces of the cell were inoculated into nude mice orthotopically. Lung node metastatic foci were obtained by culturing, the procedure was repeated for 3 cycles. Changes were observed in intra-tibial tumor growth, pulmonary metastatic rate in vivo, morphological characteristics, expressions of BMP, vimentin, actin, NEC and karyotype analyses of every passage in vitro.
The intra-tibial tumor growth rates were 100%, the proliferation abilities were stable, and the pulmonary metastatic rates were higher than 80%. The expressions of BMP, vimentin, actin were positive, but the expression of NSE was negative. Its morphological characteristics the antigen-positive substances and karyotype were similar to those observed in human osteosarcoma.
SOSP-M1 with very stable biological characteristics is a good model for the study of human osteosarcoma.
研究人高转移性骨肉瘤亚群的生物学稳定性及鉴定。
将细胞的肿瘤块原位接种于裸鼠。通过培养获得肺淋巴结转移灶，此过程重复3个周期。观察体外各代胫内肿瘤生长变化、体内肺转移率、形态学特征、骨形态发生蛋白（BMP）、波形蛋白、肌动蛋白、神经元特异性烯醇化酶（NEC）的表达及核型分析。
胫内肿瘤生长率为100%，增殖能力稳定，肺转移率高于80%。BMP、波形蛋白、肌动蛋白表达呈阳性，但神经元特异性烯醇化酶表达呈阴性。其形态学特征、抗原阳性物质及核型与人骨肉瘤相似。
具有非常稳定生物学特性的SOSP-M1是研究人骨肉瘤的良好模型。
