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构建玉米的RFLP连锁图谱并定位NCBL QTL

[Construction the RFLP linkage map and location the NCBL QTL of maize].

作者信息

Huang Lie-Jian, Xiang Dao-Quan, Yang Jun-Pin, Dai Jing-Rui

机构信息

Academy of Crop Science, China Agricultural University, Beijing 100094, China.

出版信息

Yi Chuan Xue Bao. 2002 Dec;29(12):1100-4.

Abstract

There exists the race differential phenomenon in Exserohilum turcicum (namely NCBL). At present, there are 5 identified races of NCBL and some un-known races in China. For improvement of maize resistance to NCBL, a basic method is to improve maize quantitative resistance. To identify the numbers and effects of the quantitative resistant loci to NCBL in maize, we constructed F2:3 population from resistant line P138 as male parent cross with sensitive line Z3 as female parent. Using RFLP marker technique, the RFLP linkage map of maize was cons cracted covering 1999. 8 cM, and the average distance between markers was 16.5 cM. Identified QTL of NCBL lesion length, width, and area were 3, 3, 2, respectively. The combined contribution ratios of these QTL were 58.1%, 71.5%, 27.5%, respectively. No QTL was identified NCBL lesion number per leaf, it was a trait that might be controlled by one- or oligo-genes. Finally, authors discussed and prospected the maize NCBL research in future.

摘要

玉米大斑病菌(即玉米大斑病)存在小种分化现象。目前,中国已鉴定出5个玉米大斑病菌小种以及一些未知小种。为提高玉米对玉米大斑病的抗性,一种基本方法是提高玉米的数量抗性。为鉴定玉米中对玉米大斑病数量抗性位点的数量和效应,我们以抗病自交系P138为父本、感病自交系Z3为母本构建了F2:3群体。利用RFLP标记技术,构建了覆盖1999.8 cM的玉米RFLP连锁图谱,标记间平均距离为16.5 cM。鉴定出的与玉米大斑病病斑长度、宽度和面积相关的QTL分别为3个、3个和2个。这些QTL的联合贡献率分别为58.1%、71.5%、27.5%。未鉴定出与每片叶上玉米大斑病病斑数量相关的QTL,该性状可能受一对或少数几个基因控制。最后,作者对未来玉米大斑病的研究进行了讨论和展望。

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