Tang Xingsan, Liao Yuhua, Chen Zhijian, Gao Xiang, Dong Jihua
Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(3):254-6, 259. doi: 10.1007/BF02828134.
In order to study the expression of IL-1beta and TNF-alpha in the myocardium of MCMV myocarditis and their role in the myocardial damages, 60 BALB/C mice of 4 weeks were randomly divided into two groups: 36 were injected intraperitoneally with MCMV and 24 served as control group. Immunohistochemistry was used to detect IL-1beta and TNF-alpha expression in the myocardium, and myocardial lesions were observed histopathologically. Histopathological study on the myocardium from infected mice revealed focal or diffuse lesions characterized by inflammatory cells and degeneration or necrosis of myocytes. The myocardial lesion score showed the degree of inflammatory cell infiltration was slight in MCMV myocarditis. The positive staining signals for IL-1beta and TNF-alpha proteins which mainly located in the infiltrating inflammatory cells and degenerative or necrotic myocytes were markedly detectable whereas there were no positive findings in the myocardium of control mice. IL-1beta and TNF-alpha was expressed in the myocardium of viral myocarditis murine model induced by MCMV. IL-1beta and TNF-alpha may play an important role in the pathogenesis of viral myocarditis.
为研究白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）在巨细胞病毒性心肌炎（MCMV心肌炎）小鼠心肌中的表达及其在心肌损伤中的作用，将60只4周龄的BALB/C小鼠随机分为两组：36只腹腔注射MCMV，24只作为对照组。采用免疫组织化学法检测心肌中IL-1β和TNF-α的表达，并进行组织病理学观察心肌病变。对感染小鼠心肌的组织病理学研究显示，病变呈局灶性或弥漫性，特征为炎性细胞浸润以及心肌细胞变性或坏死。心肌病变评分显示，MCMV心肌炎中炎性细胞浸润程度较轻。主要位于浸润炎性细胞及变性或坏死心肌细胞中的IL-1β和TNF-α蛋白阳性染色信号明显可检测到，而对照组小鼠心肌中未发现阳性结果。IL-1β和TNF-α在MCMV诱导的病毒性心肌炎小鼠模型心肌中表达。IL-1β和TNF-α可能在病毒性心肌炎的发病机制中起重要作用。
