Feng Su-ling, Wang Jin, Fan Jing
College of Chemistry and Environmental Science, Key Laboratory of Environmental Science and Engineering of Henan Education Department, Henan Normal University, Xinxiang 453002, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Jun;25(6):927-9.
A resonance light scattering method for the determination of trace proteins was developed. In the presence of Triton X-100, proteins reacted with Titan yellow to form a combination product, resulting a significant enhancement of resonance light scattering (RLS). The deltaI(RLS) was directly proportional to the concentration of protein in the range of 0.03-0.9 microg x mL(-1), with the detection limit 17.7 ng x mL(-1) for BSA. This method was applied to the determination of the proteins in synthetic and human serum samples, and compared to the CBB method, with satisfactory results.
