用聚甲基丙烯酸（PMAA）测定蛋白质的共振光散射技术。
Resonance light scattering technique for the determination of proteins with polymethacrylic acid (PMAA).
作者信息
Chen Yanhua, Gao Dejiang, Tian Yuan, Ai Peng, Zhang Hanqi, Yu Aimin
机构信息
College of Chemistry, Jilin University, Changchun 130012, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Jul;67(3-4):1126-30. doi: 10.1016/j.saa.2006.09.032. Epub 2006 Oct 4.
As a resonance light scattering (RLS) probe, the polyelectrolyte polymethacrylic acid (PMAA) was applied in this assay. The bovine serum albumin (BSA) and human serum albumin (HSA) were determined by the electrostatic interaction of PMAA and proteins. At pH 3.8 Na(2)HPO(4)-citric acid buffer solution, the RLS intensities of PMAA-BSA (HSA) system were greatly enhanced. The characteristic peaks were appeared at the wavelength 320, 546 and 594 nm. The optimization conditions of the reaction were also examined and selected. Under the selected conditions, the RLS intensities were proportional to the protein concentrations in the range of (0.0200-2.00) x 10(-6) mol/L for BSA and (0.0200-2.40) x 10(-6) mol/L for HSA. The influences of some foreign substances were also examined. The synthetic samples containing proteins and some real samples were analyzed and the results obtained were satisfactory.
作为一种共振光散射（RLS）探针，聚电解质聚甲基丙烯酸（PMAA）被应用于该分析方法中。通过PMAA与蛋白质的静电相互作用来测定牛血清白蛋白（BSA）和人血清白蛋白（HSA）。在pH 3.8的Na₂HPO₄ - 柠檬酸缓冲溶液中，PMAA - BSA（HSA）体系的RLS强度显著增强。在波长320、546和594 nm处出现特征峰。同时也考察并选择了反应的优化条件。在选定条件下，对于BSA，RLS强度在（0.0200 - 2.00）×10⁻⁶ mol/L范围内与蛋白质浓度成正比；对于HSA，在（0.0200 - 2.40）×10⁻⁶ mol/L范围内与蛋白质浓度成正比。还考察了一些外来物质的影响。对含有蛋白质的合成样品和一些实际样品进行了分析，所得结果令人满意。
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