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[高速紫外可见分光光度计的研制]

[Development of high speed UV-Vis spectrophotometer].

作者信息

Huang Mei-zhen, Ni Yi, Lin Feng, Yuan Bo, Dou Xiao-ming, Zhao Hai-ying, Huang Wei-shi

机构信息

Optical Engineering Lab, Physics Department, Shanghai Jiaotong University, Shanghai 200240, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Jun;25(6):938-41.

PMID:16201377
Abstract

A high speed spectrophotometer based on a linear CCD was developed. The function and structure of the hardware and the software for the instrument were discussed. The elementary performance was tested as follows: wavelength range 200-820 nm, full spectrum scan time < 0.1 s, spectral bandwidth 0.7 nm, wavelength accuracy +/-1 nm, photometric accuracy +/- 0.005 AU, baseline flatness < 0.001 AU (rms), and stray light < 0.1%. Results showed that this instrument is easy to use, fast and with high analytical performance. In addition, it has internet function of remote control and remote access. With the powerful data processing and spectrum analyzing software, it will provide a strong tool for routine analysis and the research on dynamic reaction in chemistry and biology fields.

摘要

研制了一种基于线性电荷耦合器件(CCD)的高速分光光度计。讨论了该仪器硬件和软件的功能与结构。对其基本性能进行了如下测试:波长范围200 - 820纳米,全光谱扫描时间<0.1秒,光谱带宽0.7纳米,波长准确度±1纳米,光度准确度±0.005吸光度单位(AU),基线平整度<0.001 AU(均方根),杂散光<0.1%。结果表明,该仪器使用方便、速度快且具有高分析性能。此外,它具有远程控制和远程访问的网络功能。借助功能强大的数据处理和光谱分析软件,它将为化学和生物学领域的常规分析及动态反应研究提供有力工具。

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