一台微型快速扫描分光光度计。

A mini-rapid-scan-spectrophotometer.

作者信息

Schmidt Werner

机构信息

Fachbereich Biologie Philipps-Universität Marburg, Karl-von-Frischstr. 8, D-35032 Marburg, Lahnberge, Germany.

出版信息

J Biochem Biophys Methods. 2004 Feb 27;58(2):125-37. doi: 10.1016/j.jbbm.2003.10.003.

DOI:10.1016/j.jbbm.2003.10.003

PMID:14980786

Abstract

The mini-rapid-scan-spectrophotometer (Mini-RSS) is a scanning single-beam spectrophotometer that has been patented. It is based on a minimum of reflections and involves exclusively mirrors as beam-deflecting components. This way stray light is minimized, which results in an excellent light-throughput, high dynamics, low cost, compactness and rigidity. The Mini-RSS has been designed as a multi-purpose instrument that allows absorption, transmission, reflection, fluorescence and luminescence measurements in a single-beam mode. Its spectral range extends from the UV and visible spectrum to the IR. This provides for the possibility to measure even optically unfavorable, highly turbid or scattering samples that would be otherwise inaccessible to investigations with commercial spectrophotometers. A miniaturized and very sensitive photomultiplier-module (PM) of high dynamics allows in the visible spectral range absorbance measurements that cover up to four OD units. The Mini-RSS is capable of scanning up to 100 spectra per second with a resolution of 12 bit and 500 points. The linear dispersion is currently 5 nm and the stray light level <0.01%.

摘要

微型快速扫描分光光度计（Mini-RSS）是一种已获专利的扫描单光束分光光度计。它基于最少的反射，仅使用镜子作为光束偏转部件。通过这种方式，杂散光被降至最低，从而实现了出色的光通量、高动态范围、低成本、紧凑性和刚性。Mini-RSS被设计为一种多功能仪器，可在单光束模式下进行吸收、透射、反射、荧光和发光测量。其光谱范围从紫外和可见光谱延伸至红外光谱。这使得即使是光学性质不佳、高度浑浊或散射的样品也能够被测量，而这些样品用商业分光光度计进行研究时是无法测量的。一个小型化且非常灵敏的高动态范围光电倍增管模块（PM）使得在可见光谱范围内能够进行高达四个光密度单位的吸光度测量。Mini-RSS能够以12位分辨率和500个点每秒扫描多达100个光谱。目前线性色散为5纳米，杂散光水平<0.01%。