[DNA-protein interactions in chromatin particles differing in electrophoretic mobility].

The chromatin particles from Ehrlich carcinoma differing in the electrophoretic mobility were divided to a transcription active (c-particles) and transcription inactive (a-particles) fractions. Analysis of the DNA-protein interaction strength by the nucleoprotein-celite-chromatography has demonstrated that the majority of DNA-protein relationships in the a-particles is destroyed at NaCl concentrations exceeding 2 M, while in the c-particles at 1 M of NaCl. The study of NPC-chromatographic position of DNA depending on the particle size has shown that in slightly fragmented by streptococcal nuclease preparations of chromatin the DNA-protein relationships are destroyed at 3 M and 1 M of NaCl. Nevertheless, the position of the DNA spike on the chromatogram is not definitely dependent on the particle size.