RNA聚合酶II亚基Rpb7促进着丝粒转录和RNA干扰介导的染色质沉默。
RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.
作者信息
Djupedal Ingela, Portoso Manuela, Spåhr Henrik, Bonilla Carolina, Gustafsson Claes M, Allshire Robin C, Ekwall Karl
机构信息
Karolinska Institutet, Department of Biosciences, School of Life Sciences, University College Sodertorn, S-141 89 Huddinge, Sweden.
出版信息
Genes Dev. 2005 Oct 1;19(19):2301-6. doi: 10.1101/gad.344205.
Abstract
Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed chromatin modification but did not affect pre-siRNA levels. Here we show that another Pol II subunit, Rpb7 has a specific role in pre-siRNA transcription. We define a centromeric pre-siRNA promoter from which initiation is exquisitely sensitive to the rpb7-G150D mutation. In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing.
摘要
裂殖酵母着丝粒重复序列被转录成小干扰RNA（siRNA）前体（pre-siRNA），这些前体由Dicer加工以指导异染色质形成。最近，RNA聚合酶II（RNA Pol II）的Rpb1和Rpb2亚基被证明介导RNA干扰（RNAi）导向的染色质修饰，但不影响pre-siRNA水平。在此我们表明，另一个Pol II亚基Rpb7在pre-siRNA转录中具有特定作用。我们定义了一个着丝粒pre-siRNA启动子，从该启动子起始对rpb7-G150D突变极为敏感。与其他Pol II亚基不同，Rpb7促进RNAi导向的染色质沉默所需的pre-siRNA转录。
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