• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

检测栽培担子菌金针菇（金针菇）退化菌株的简易比色法。

Simple colorimetric method for detecting degenerate strains of the cultivated basidiomycete Flammulina velutipes (Enokitake).

作者信息

Magae Yumi, Akahane Kobun, Nakamura Kimiyoshi, Tsunoda Shigeyuki

机构信息

Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.

出版信息

Appl Environ Microbiol. 2005 Oct;71(10):6388-9. doi: 10.1128/AEM.71.10.6388-6389.2005.

DOI:10.1128/AEM.71.10.6388-6389.2005
PMID:16204563
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1266012/
Abstract

Degeneration of cultivated strains of Flammulina velutipes is a serious problem. We developed a simple colorimetric method to detect degenerate strains by using a liquid medium supplemented with bromothymol blue and lactose. The ability of a strain to develop normal mushrooms could be determined by the color of the medium.

摘要

金针菇栽培菌株的退化是一个严重问题。我们开发了一种简单的比色法，通过使用添加了溴百里酚蓝和乳糖的液体培养基来检测退化菌株。菌株形成正常子实体的能力可以通过培养基的颜色来确定。

相似文献

1
Simple colorimetric method for detecting degenerate strains of the cultivated basidiomycete Flammulina velutipes (Enokitake).检测栽培担子菌金针菇（金针菇）退化菌株的简易比色法。
Appl Environ Microbiol. 2005 Oct;71(10):6388-9. doi: 10.1128/AEM.71.10.6388-6389.2005.
2
Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display.利用荧光差异显示技术分离与分析金针菇子实体发育过程中特异表达的基因
FEMS Microbiol Lett. 2006 Jan;254(1):165-72. doi: 10.1111/j.1574-6968.2005.00023.x.
3
Relationship between fruiting body development and extracellular laccase production in the edible mushroom .食用菌子实体发育与胞外漆酶产生之间的关系
Biochem Biophys Rep. 2022 Jan 10;29:101204. doi: 10.1016/j.bbrep.2022.101204. eCollection 2022 Mar.
4
Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake).可食用担子菌金针菇中的双链RNA和病毒样颗粒。
FEMS Microbiol Lett. 1999 Nov 15;180(2):331-5. doi: 10.1111/j.1574-6968.1999.tb08814.x.
5
Comparative Proteome Reveals Metabolic Changes during the Fruiting Process in Flammulina velutipes.比较蛋白质组揭示金针菇子实体形成过程中的代谢变化。
J Agric Food Chem. 2017 Jun 21;65(24):5091-5100. doi: 10.1021/acs.jafc.7b01120. Epub 2017 Jun 12.
6
Compositional analysis of the fruiting body of transgenic Flammulina velutipes producing resveratrol.产白藜芦醇的转基因金针菇子实体的成分分析。
Food Chem. 2014 Dec 1;164:211-8. doi: 10.1016/j.foodchem.2014.05.023. Epub 2014 May 16.
7
A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom ().单一转录因子（PDD1）决定冬季蘑菇的发育和产量（）。
Appl Environ Microbiol. 2019 Nov 27;85(24). doi: 10.1128/AEM.01735-19. Print 2019 Dec 15.
8
A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes.一种与jacalin相关的凝集素调控金针菇气生菌丝体和子实体的形成。
Int J Mol Sci. 2016 Nov 28;17(12):1884. doi: 10.3390/ijms17121884.
9
Lipidomic Profiling of (Curtis) Singer (Agaricomycetes) through Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry: Examining Lipid Dynamics Changes during Fruiting Body Formation and Development.采用超高效液相色谱-四极杆飞行时间质谱法对（Curtis）Singer（伞菌目）进行脂质组学分析：研究子实体形成和发育过程中脂质动态变化。
J Agric Food Chem. 2024 Aug 14;72(32):18271-18282. doi: 10.1021/acs.jafc.4c03863. Epub 2024 Jul 29.
10
Detrimental and essential elements in fruiting bodies of wild-growing fungi , , and .野生真菌子实体中的有害和有益元素 ,, 和 。
J Environ Sci Health B. 2022;57(4):243-251. doi: 10.1080/03601234.2022.2046994. Epub 2022 Mar 3.

引用本文的文献

1
Comparative transcriptomics and metabolomics provide insight into degeneration-related physiological mechanisms of Morchella importuna after long-term preservation.比较转录组学和代谢组学为深入了解长期保存后皱柄羊肚菌的退化相关生理机制提供了见解。
Microb Biotechnol. 2025 Jan;18(1):e70045. doi: 10.1111/1751-7915.70045.
2
Exogenous MnSO Improves Productivity of Degenerated by Regulating Antioxidant Activity.外源性硫酸锰通过调节抗氧化活性提高退化组织的生产力。 （注：原文中“Degenerated”后缺少“tissues”之类的词，表述不太完整准确，但按要求翻译如上）
J Fungi (Basel). 2024 Nov 27;10(12):825. doi: 10.3390/jof10120825.
3
Nondestructive detection of strain degradation based on micro-hyperspectral imaging and machine learning.基于显微高光谱成像和机器学习的应变降解无损检测
Front Plant Sci. 2023 Dec 6;14:1260625. doi: 10.3389/fpls.2023.1260625. eCollection 2023.
4
The phenomenon of strain degeneration in biotechnologically relevant fungi.生物技术相关真菌中的菌株退化现象。
Appl Microbiol Biotechnol. 2023 Aug;107(15):4745-4758. doi: 10.1007/s00253-023-12615-z. Epub 2023 Jun 21.
5
Relationship between fruiting body development and extracellular laccase production in the edible mushroom .食用菌子实体发育与胞外漆酶产生之间的关系
Biochem Biophys Rep. 2022 Jan 10;29:101204. doi: 10.1016/j.bbrep.2022.101204. eCollection 2022 Mar.
6
Label-Free Proteomics Reveals the Molecular Mechanism of Subculture Induced Strain Degeneration and Discovery of Indicative Index for Degeneration in .无标记蛋白质组学揭示传代诱导的菌株退化的分子机制及退化指示性指标的发现。
Molecules. 2020 Oct 24;25(21):4920. doi: 10.3390/molecules25214920.
7
Characteristics Analysis Reveals the Progress of Mycelium Subculture Degeneration.特征分析揭示了菌丝体继代培养退化的进程。
Front Microbiol. 2019 Sep 3;10:2045. doi: 10.3389/fmicb.2019.02045. eCollection 2019.
8
Comparative Transcriptomic Analysis of MAPK-Mediated Regulation of Sectorization in Cryphonectria parasitica.比较转录组分析 MAPK 介导的Cryphonectria parasitica 分域调控
Mol Cells. 2019 Apr 30;42(4):363-375. doi: 10.14348/molcells.2019.0019.
9
Grouping of multicopper oxidases in Lentinula edodes by sequence similarities and expression patterns.香菇中多铜氧化酶的序列相似性和表达模式聚类。
AMB Express. 2015 Dec;5(1):63. doi: 10.1186/s13568-015-0151-2. Epub 2015 Sep 17.
10
Identification of degenerate nuclei and development of a SCAR marker for Flammulina velutipes.金针菇退化细胞核的鉴定及SCAR标记的开发
PLoS One. 2014 Sep 15;9(9):e107207. doi: 10.1371/journal.pone.0107207. eCollection 2014.

本文引用的文献

1
Inheritance of Strain Instability (Sectoring) in the Commercial Button Mushroom, Agaricus bisporus.商业纽扣蘑菇（双孢蘑菇）菌株不稳定性（扇形）的遗传。
Appl Environ Microbiol. 1994 Jul;60(7):2384-8. doi: 10.1128/aem.60.7.2384-2388.1994.
2
Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake).可食用担子菌金针菇中的双链RNA和病毒样颗粒。
FEMS Microbiol Lett. 1999 Nov 15;180(2):331-5. doi: 10.1111/j.1574-6968.1999.tb08814.x.