Chen Xiao, Zhang Zheng, Liu Xiaoxia, Cui Bo, Miao Wentao, Cheng Weiwei, Zhao Fengyun
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
School of Food Science and Technology, Qilu University of Technology, Jinan, China.
Front Microbiol. 2019 Sep 3;10:2045. doi: 10.3389/fmicb.2019.02045. eCollection 2019.
is a typical edible Basidiomycete with a high-temperature tolerance. It has a strong fibrinolysis capability and consumes abundant agricultural wastes. In agricultural cultivation, mycelial subculturing has been adopted, leading to serious strain degeneration. In this study, continuous mycelial subculturing of the common strain V971 (original strain recorded as M0) was performed in potato dextrose agar (PDA) medium. One generation of the strain was preserved every 3 months (90 days); thus, six generations of degenerated strains (M1-M6) were obtained after 18 months of mycelial subculturing. The original and degenerated strains were preserved in sterile paraffin liquid at room temperature (18-25°C). The biological traits and nutrients of M0 and M1-M6 were studied. The mycelial growth rate and biomass initially increased and then decreased as the degeneration progressed, reaching minimum levels of 0.041 ± 0.001 cm/h and 1.82 ± 0.25 g, respectively, at M6. Additionally, the polysaccharide, protein, polyphenol, flavone, total amino acid, and total mineral element contents of the strains decreased continuously, reaching minimum levels of 30.12 ± 3.12 g/100 g, 26.42 ± 2.1 g/100 g, 1.08 ± 0.05 g/100 g, 4.23 ± 0.21 g/100 g, 12.51 mg/g, and 398.05 mg/kg, respectively, at M6. The decolorization capability of in liquid medium supplemented with bromothymol blue and lactose reflected the degree of strain degeneration, with the capability weakening as the degeneration intensified. These results are highly significant for production. The mycelial characteristics during subculture-associated degeneration were described and provide an early identification method for 's degeneration.
是一种典型的耐高温食用担子菌。它具有很强的纤溶能力，能消耗大量农业废弃物。在农业栽培中，已采用菌丝体继代培养，但导致了严重的菌株退化。本研究在马铃薯葡萄糖琼脂（PDA）培养基中对常见菌株V971（原始菌株记录为M0）进行连续菌丝体继代培养。每3个月（90天）保存一代菌株；因此，经过18个月的菌丝体继代培养后，获得了六代退化菌株（M1 - M6）。原始菌株和退化菌株保存在室温（18 - 25°C）的无菌石蜡液中。对M0和M1 - M6的生物学特性和营养成分进行了研究。随着退化的进行，菌丝体生长速率和生物量最初增加然后下降，在M6时分别达到最低水平0.041±0.001 cm/h和1.82±0.25 g。此外，菌株的多糖、蛋白质、多酚、黄酮、总氨基酸和总矿质元素含量持续下降，在M6时分别达到最低水平30.12±3.12 g/100 g、26.42±2.1 g/100 g、1.08±0.05 g/100 g、4.23±0.21 g/100 g、12.51 mg/g和398.05 mg/kg。在添加溴百里酚蓝和乳糖的液体培养基中的脱色能力反映了菌株退化程度，随着退化加剧能力减弱。这些结果对……生产具有高度重要意义。描述了继代培养相关退化过程中的菌丝体特征，并为……的退化提供了一种早期鉴定方法。 （注：原文中部分地方“……”指代不明，翻译时保留原样）
