Wen Li-Yong, Chen Jun-Hu, Ding Jian-Zu, Zhang Jian-Feng, Lu Shao-Hong, Yu Li-Ling, Shen Li-Ying, Wu Guan-Ling, Zhou Xiao-Nong, Zheng Jiang
Research Laboratory of Molecular Immunoparasitology, Nanjing Medical University, Nanjing 210029, China.
Acta Trop. 2005 Nov-Dec;96(2-3):142-7. doi: 10.1016/j.actatropica.2005.07.025. Epub 2005 Oct 3.
The dot immunogold filtration assay (DIGFA) is a rapid technique for the detection of anti-Schistosoma japonicum antibody. Its sensitivity with regard to sera obtained from patients with acute or chronic schistosomiasis was shown to be 100 and 96.9%, respectively. The specificity when using sera of people living in an area non-endemic for schistosomiasis japonica was 100%. Cross-reaction rates for paragonimiasis and clonorchiasis patients were 14.3% and 0%, respectively. Parallel serum tests of 1091 residents from an area endemic for S. japonicum by means of DIGFA, enzyme-linked immunosorbent assay and indirect haemagglutination test resulted in positive rates of 9.3%, 11.5% and 11.0%, respectively. Thus, there was a high level of agreement between the sets of results (P>0.05). In conclusion, DIGFA holds considerable promise for rapid and accurate diagnosis of S. japonicum, as it does not require any specific instruments and can be applied with ease. DIGFA has therefore several advantages over conventional diagnostic approaches and is useful not only for screening and sero-epidemiological surveys in the field, but also in clinical settings.
斑点免疫金渗滤法（DIGFA）是一种检测日本血吸虫抗体的快速技术。结果显示，该方法对急性或慢性血吸虫病患者血清的检测灵敏度分别为100%和96.9%。在使用日本血吸虫病非流行区人群血清时，其特异性为100%。肺吸虫病和华支睾吸虫病患者的交叉反应率分别为14.3%和0%。采用DIGFA、酶联免疫吸附试验和间接血凝试验对1091名日本血吸虫病流行区居民进行血清平行检测，阳性率分别为9.3%、11.5%和11.0%。因此，各组结果之间具有高度一致性（P>0.05）。总之，DIGFA在快速、准确诊断日本血吸虫病方面具有很大潜力，因为它不需要任何特殊仪器，且易于应用。因此，DIGFA相对于传统诊断方法具有多个优势，不仅可用于现场筛查和血清流行病学调查，也适用于临床环境。
