Yisheng Liu, Wenping Du, Kuiyang Zheng, Linlin Fu, Ming Chen
Department of Parasitology, Xuzhou Medical College, Xuzhou, Jiangsu, PR China.
Southeast Asian J Trop Med Public Health. 2005 Jan;36(1):79-82.
A fast, specific, sensitive, convenient, and economical rapid-dot-immunogold staining (R-Dot-IGS) assay was used to detect serum antibodies in patients infected with Schistosoma japonicum. The soluble egg antigen of Schistosoma japonicum was added onto microspore membrane. After pre-reacting and blocking, the serum to be detected and sheep anti-human IgG labeled with chloroauric acid were added sequentially. The assay took 15 minutes. For comparison, the dot-immunogold silver staining (Dot-IGSS) and rapid micro-volume Dot-IGSS (RM-Dot-IGSS) assay were also performed. The positive rate to detect the serum of schistosomiasis japonica by the R-Dot-IGS, Dot-IGSS and RM-Dot-IGSS assay was 98%, 98% and 100%, respectively. Samples from 50 healthy controls, 10 cases of clonorchiasis, and 10 cases of paragonimiasis showed negative reactions except for one case of clonorchiasis with RM-Dot-IGSS assay. Compared with Dot-IGSS and RM-Dot-IGSS, R-Dot-IGS assay has similar sensitivity and specificity, but the latter is quicker, simpler, and cheaper. Therefore, R-Dot-IGS is strongly recommended for rapid diagnosis of schistosomiasis japonica both in epidemiological study and in the clinic.
采用一种快速、特异、灵敏、便捷且经济的快速斑点免疫金染色（R-Dot-IGS）检测法检测日本血吸虫感染患者的血清抗体。将日本血吸虫可溶性虫卵抗原滴加于微孔滤膜上。经预反应及封闭后，依次加入待检血清和氯金酸标记的羊抗人IgG。该检测法耗时15分钟。作为对照，还进行了斑点免疫金银染色（Dot-IGSS）及快速微量斑点免疫金银染色（RM-Dot-IGSS）检测。R-Dot-IGS、Dot-IGSS及RM-Dot-IGSS检测法检测日本血吸虫病血清的阳性率分别为98%、98%和100%。50份健康对照、10份华支睾吸虫病患者及10份肺吸虫病患者样本，除1份华支睾吸虫病样本采用RM-Dot-IGSS检测法出现阳性外，其余均呈阴性反应。与Dot-IGSS及RM-Dot-IGSS相比，R-Dot-IGS检测法具有相似的敏感性和特异性，但前者更快、更简便且更经济。因此，强烈推荐R-Dot-IGS用于日本血吸虫病流行病学研究及临床的快速诊断。
