Vega Yolanda, Pérez-Alvárez Lucía, Delgado Elena, Muñoz Mercedes, Casado Gema, Carmona Rocío, Sierra María, Vázquez de Parga Elena, Pinilla Milagros, García Valentina, Medrano Leandro, Contreras Gerardo, Thomson Miguel, Nájera Rafael
Area de Patogenia Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
J Clin Microbiol. 2005 Oct;43(10):5301-4. doi: 10.1128/JCM.43.10.5301-5304.2005.
The oligonucleotide ligation assay is a genotypic assay for the detection of resistance-associated mutations to reverse transcriptase and protease inhibitors in human immunodeficiency virus type 1 subtype B. This assay has been modified and developed for non-B subtypes and recombinant strains and has been evaluated with sequencing, resulting in a more sensitive assay than sequencing for non-B subtypes.
寡核苷酸连接测定法是一种用于检测人类免疫缺陷病毒1型B亚型中逆转录酶和蛋白酶抑制剂耐药相关突变的基因分型测定法。该测定法已针对非B亚型和重组毒株进行了改进和开发,并已通过测序进行评估,结果表明对于非B亚型,该测定法比测序更灵敏。