Hawrot Małgorzata, Nowak Andrzej, Kłódka Dariusz
Department of Microbiology and Environmental Biotechnology, University of Agriculture, Slowackiego 17, 71-434 Szczecin, Poland.
Pol J Microbiol. 2005;54(1):49-53.
Activity of the enzymes can give information on the kind and duration of the effects of pollutants on the metabolic activity of soil. Results of studies on the dehydrogenase activity in soils experimentally contaminated with diesel fuel, with and without modification used in investigation over biodegradation process (including fertilization, stirring and inoculation) were presented in paper. Studies with two rates of pollution were carried out for soils of various organic matter content and different soil particle size grading. Dehydrogenase activity was determined with TTC (2,3,5-triphenyltetrazolium chloride) as a substrate. Increase of dehydrogenase activity was found in the soils that were treated with diesel fuel. Regardless of pollution rate, usually higher activity was recorded in sandy soil. Variance analysis confirmed the highly significant influence of applied modifications on increase of the enzymatic activity in relation to not modified object.
酶的活性可以提供有关污染物对土壤代谢活性影响的类型和持续时间的信息。本文展示了对受柴油污染的土壤中脱氢酶活性的研究结果，该研究采用了不同的处理方式（包括施肥、搅拌和接种）来研究生物降解过程，研究针对不同有机质含量和不同土壤颗粒大小分级的土壤设置了两种污染水平。以TTC（2,3,5-三苯基氯化四氮唑）为底物测定脱氢酶活性。结果发现，用柴油处理过的土壤中脱氢酶活性有所增加。无论污染程度如何，通常沙质土壤中的活性更高。方差分析证实，与未处理的对象相比，所应用的处理方式对酶活性的增加具有极显著影响。
