Abbondanzi F, Antonellini R, Campisi T, Gagni S, Malaspina F, Iacondini A
Centro Ricerche Ambientali Montecatini, Via Ciro Menotti 48, 48023 Marina di Ravenna, RA.
Ann Chim. 2001 Jul-Aug;91(7-8):391-400.
This study focused on the impact of a recent soil pollution of diesel fuel on a site and its indigenous microbial flora. A pilot plant (0.5 m3) was set up and filled with a soil (about 700 kg), artificially and uniformly polluted with diesel fuel (7 g/kg). This plant was then chemically and biologically monitored during the whole experiment (about two years). During the monitoring, a morphological change of the microbial colonies was observed. This was probably due to the acclimation phenomena to the pollution. With batch kinetic studies (10 ml) and increasing the selective pressure of the pollutant, it was possible to select and isolate a microbial consortium and a single strain that developed the ability to use different diesel fuel fractions as carbon sources. GC-MS analytical techniques were used. Results showed that different fractions were degraded at different times. In the batch system, in 7 days, the microbial consortium degraded some aromatic hydrocarbons. The isolate strain, in 20 days, degraded linear hydrocarbons. After a two years acclimation, it was possible to obtain, from a pilot plant, a microbial consortium and a strain able to degrade diesel fuel, for a future bioremediation in situ process.
本研究聚焦于近期柴油对某场地土壤污染及其本土微生物群落的影响。搭建了一个中试装置（0.5立方米），装入约700千克人工均匀污染了柴油（7克/千克）的土壤。在整个实验过程（约两年）中，对该装置进行化学和生物学监测。监测期间，观察到微生物菌落的形态变化。这可能是由于对污染的驯化现象所致。通过批次动力学研究（10毫升）并增加污染物的选择压力，能够筛选并分离出一个微生物群落和一个单一菌株，它们具备利用不同柴油馏分作为碳源的能力。使用了气相色谱 - 质谱联用（GC - MS）分析技术。结果表明，不同馏分在不同时间被降解。在批次系统中，7天内微生物群落降解了一些芳烃。分离出的菌株在20天内降解了直链烃。经过两年的驯化，从中试装置中获得了一个能够降解柴油的微生物群落和菌株，用于未来的原位生物修复过程。
