Winstanley F P, Eckersall P D
Institute of Biochemistry, Glasgow Royal Infirmary.
Res Vet Sci. 1992 May;52(3):273-6. doi: 10.1016/0034-5288(92)90024-v.
A bioassay for bovine interleukin-1 (IL1) activity is described. The assay is based on the IL1-stimulated proliferation of a mouse T-lymphocyte cell line, D10(N4)M. Bovine mononuclear cells stimulated with lipopolysaccharide produce an interleukin-1-like activity which stimulated the growth of the D10(N4)M cell line in a dose-dependent manner. The stimulatory activity was neutralised by a combination of both anti-human IL1 alpha and anti-human IL1 beta sera. The quantity of IL1-like activity released from the mononuclear cells increased asymptotically with increasing lipopolysaccharide dose.
本文描述了一种牛白细胞介素-1（IL1）活性的生物测定法。该测定法基于IL1刺激的小鼠T淋巴细胞系D10(N4)M的增殖。用脂多糖刺激的牛单核细胞产生一种白细胞介素-1样活性，该活性以剂量依赖的方式刺激D10(N4)M细胞系的生长。抗人IL1α和抗人IL1β血清的组合可中和刺激活性。单核细胞释放的IL1样活性的量随着脂多糖剂量的增加而渐近增加。
