Ennist D L, Greenblatt D, Coffman R, Sharma S, Maizel A, Howard M
Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Cell Immunol. 1987 Nov;110(1):77-94. doi: 10.1016/0008-8749(87)90103-1.
To further characterize a human B-cell growth factor (BCGF) produced by phytohemagglutinin (PHA) P-stimulated peripheral blood T cells, a partially purified preparation of this material was tested in a number of murine assays for B-cell stimulatory factors (BSF). Human BCGF lacked murine BSF-1 activity as assessed via the induction of polyclonal proliferation of anti-IgM-stimulated murine B cells; however, this material consistently augmented the proliferative response of murine B cells to anti-IgM and a saturating dose of murine BSF-1. Human BCGF also induced proliferation in unstimulated murine B cells, and augmented the proliferative response of dextran sulfate activated murine B cells. Human BCGF is therefore capable of causing proliferation of unstimulated and activated murine B cells, and by these criteria closely resembles murine BCGF II. In contrast to murine BCGF II, however, human BCGF failed to stimulate proliferation or immunoglobulin (Ig) secretion by murine BCL1 B lymphoma cells. A murine analog of this human BCGF showing the same pattern of biological responses was found in concanavalin A-stimulated supernatants of the murine MB2.1 T-cell line and D9-Cl T-cell hybridoma. The active component of the human BCGF preparation was not due to contaminating PHA, interleukin 1, interleukin 2; interferon-gamma, or endotoxin. Comparison between the above human BCGF and a commonly used source of murine BCGF II, i.e., supernatant from antigen-stimulated D10.G4.1 T cells, provided information suggestive of BCGF II heterogeneity. Both human BCGF and D10.G4.1 supernatant caused proliferation of unstimulated and dextran sulfate-stimulated murine B cells; however, only the human BCGF preparation augmented the proliferative response of murine B cells to anti-IgM and a saturating dose of murine BSF-1, and only the D10.G4.1 supernatant stimulated BCL1 cell proliferation and immunoglobulin secretion. The data therefore indicate that the different assays for BCGF II used in this study respond to different factors, and suggest the existence of two BCGF II-like activities.
为了进一步表征由植物血凝素（PHA）P刺激的外周血T细胞产生的人B细胞生长因子（BCGF），在一些针对B细胞刺激因子（BSF）的小鼠试验中对这种物质的部分纯化制剂进行了测试。通过抗IgM刺激的小鼠B细胞的多克隆增殖诱导评估，人BCGF缺乏小鼠BSF-1活性；然而，这种物质始终增强了小鼠B细胞对抗IgM和饱和剂量小鼠BSF-1的增殖反应。人BCGF还诱导未刺激的小鼠B细胞增殖，并增强硫酸葡聚糖激活的小鼠B细胞的增殖反应。因此，人BCGF能够引起未刺激和激活的小鼠B细胞增殖，根据这些标准，它与小鼠BCGF II非常相似。然而，与小鼠BCGF II不同的是，人BCGF未能刺激小鼠BCL1 B淋巴瘤细胞的增殖或免疫球蛋白（Ig）分泌。在伴刀豆球蛋白A刺激的小鼠MB2.1 T细胞系和D9-Cl T细胞杂交瘤的上清液中发现了具有相同生物学反应模式的这种人BCGF的小鼠类似物。人BCGF制剂的活性成分不是由于污染的PHA、白细胞介素1、白细胞介素2、干扰素-γ或内毒素。上述人BCGF与常用的小鼠BCGF II来源（即抗原刺激的D10.G4.1 T细胞的上清液）之间的比较提供了提示BCGF II异质性的信息。人BCGF和D10.G4.1上清液都引起未刺激和硫酸葡聚糖刺激的小鼠B细胞增殖；然而，只有人BCGF制剂增强了小鼠B细胞对抗IgM和饱和剂量小鼠BSF-1的增殖反应，只有D10.G4.1上清液刺激BCL1细胞增殖和免疫球蛋白分泌。因此，数据表明本研究中用于BCGF II的不同试验对不同因子有反应，并提示存在两种BCGF II样活性。
