Wang Yueju, Balgley Brian M, Lee Cheng S
Calibrant Biosystems, Gaithersburg, MD 20878, USA.
Expert Rev Proteomics. 2005 Oct;2(5):659-67. doi: 10.1586/14789450.2.5.659.
The capabilities of capillary isoelectric focusing-based multidimensional separations for performing proteome analysis from minute samples create new opportunities in the pursuit of biomarker discovery using enriched and selected cell populations procured from tissue specimens. In this article, recent advances in online integration of capillary isoelectric focusing with nano-reversed phase liquid chromatography for achieving high-resolution peptide and protein separations prior to mass spectrometry analysis are reviewed, along with its potential application to tissue proteomics. These proteome technological advances combined with recently developed tissue microdissection techniques, provide powerful tools for those seeking to gain a greater understanding at the global level of the cellular machinery associated with human diseases such as cancer.
基于毛细管等电聚焦的多维分离技术能够从微量样本中进行蛋白质组分析，这为利用从组织标本中获取的富集和选定细胞群体来寻找生物标志物创造了新机会。本文综述了毛细管等电聚焦与纳米反相液相色谱在线联用技术在质谱分析前实现高分辨率肽和蛋白质分离方面的最新进展，以及其在组织蛋白质组学中的潜在应用。这些蛋白质组学技术进展与最近开发的组织显微切割技术相结合，为那些试图在全球层面更深入了解与癌症等人类疾病相关的细胞机制的人提供了强大工具。
