Qian Wei-Jun, Camp David G, Smith Richard D
Biological Science Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Expert Rev Proteomics. 2004 Jun;1(1):87-95. doi: 10.1586/14789450.1.1.87.
The advent of high-throughput proteomic technologies for global detection and quantitation of proteins creates new opportunities and challenges for those seeking to gain greater understanding of the cellular machinery. Here, recent advances in high-resolution capillary liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry are reviewed along with its potential application to high-throughput proteomics. These technological advances combined with quantitative stable isotope labeling methodologies provide powerful tools for expanding our understanding of biology at the system level.
用于蛋白质全局检测和定量的高通量蛋白质组学技术的出现，为那些寻求更深入了解细胞机制的人带来了新的机遇和挑战。本文综述了高分辨率毛细管液相色谱与傅里叶变换离子回旋共振质谱联用技术的最新进展及其在高通量蛋白质组学中的潜在应用。这些技术进步与定量稳定同位素标记方法相结合，为在系统水平上扩展我们对生物学的理解提供了强大工具。
