Kossoy George, Ben-Hur Herzl, Elhayany Asher, Schneider David F, Kossoy Nadja, Zusman Itshak
Laboratory of Experimental Medicine, Park Rabin, Rehovot, Israel.
Oncol Rep. 2005 Nov;14(5):1317-21.
Previously, we showed that the 66 and 51 kDa soluble tumor-associated antigens (sTAAs) have distinct suppressive effects on chemically induced mammary cancer in rats, both alone and in combination with the hormone-related anticancer drug tamoxifen. Here, we describe the effects of both sTAA and tamoxifen on the histological structure of ovaries in mammary tumor-bearing 30- to 34-week-old rats. Central ovary sections were pooled, the number of the healthy and degenerated follicles were counted, and the size of the corpora lutea was estimated. In follicular development primordial, primary, preantral and antral stages were recognized. Only healthy follicles with visible nuclei were counted. Follicular degeneration was estimated as the number of atretic follicles with follicular remnants. Treatment with tamoxifen alone or in combination with sTAA significantly increased the number of primordial follicles and atretic follicles in the ovaries, and promoted the formation of small follicular cysts. Total area of the corpora lutea decreased. sTAA participated in this process by increasing apoptosis in degenerated follicles.
此前，我们发现66 kDa和51 kDa的可溶性肿瘤相关抗原（sTAA）对化学诱导的大鼠乳腺癌具有不同的抑制作用，无论是单独使用还是与激素相关抗癌药物他莫昔芬联合使用。在此，我们描述了sTAA和他莫昔芬对30至34周龄患乳腺肿瘤大鼠卵巢组织结构的影响。收集卵巢中央部分切片，计数健康卵泡和退化卵泡的数量，并估算黄体大小。在卵泡发育过程中，识别出原始卵泡、初级卵泡、窦前卵泡和窦状卵泡阶段。仅对具有可见细胞核的健康卵泡进行计数。卵泡退化以带有卵泡残余物的闭锁卵泡数量来估算。单独使用他莫昔芬或与sTAA联合使用均显著增加了卵巢中原始卵泡和闭锁卵泡的数量，并促进了小卵泡囊肿的形成。黄体总面积减小。sTAA通过增加退化卵泡中的细胞凋亡参与了这一过程。
