Aljassim Fatimah Khalil, El-Sheikh Amal Ahmed, Motabagani Mohamed A
Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
J Microsc Ultrastruct. 2022 Nov 9;12(1):1-5. doi: 10.4103/jmau.jmau_79_21. eCollection 2024 Jan-Mar.
Tamoxifen is a drug that has been used extensively as a chemotherapeutic agent for breast cancer. It should be taken for a long period, from few weeks up to many years, so it can induce gynecological and nongynecological complications.
Present study was conducted to clarify the histopathological effects of tamoxifen intake on the ovarian follicles of rats and evaluate the promising recovery after drug withdrawal.
Adult female albino rats ( = 24) were randomly divided into four groups. Group I: Control rats without treatment. Group II: Rats received olive oil vehicle. Group III: Rats received 5 mg/kg daily of tamoxifen dissolved in olive oil by oral administration for 4 weeks. Group IV: Rats received tamoxifen as in Group III then will be kept for another 4 weeks without treatment for recovery. Then, the rats were anaesthetized and the ovaries were removed and prepared for histological assessment by light microscope.
The ovarian histological findings in the ovary of Group III revealed an increase in atretic ovarian follicles, appearance of cystic ovarian follicles, and cystic corpus luteum. The granulosa cells of ovarian follicles were disorganized with vacuolation of their cytoplasm, increased number of pyknotic nuclei, fragmented nuclei, and apoptotic bodies. After the withdrawal of drug, the ovarian tissue showed slight improvement with the appearance of some atretic follicles with degenerated oocyte and stromal hyperplasia.
Based on the results, tamoxifen induced marked histological changes in the ovary. If tamoxifen is mandatory for the prevention of breast cancer, frequent gynecological examination should be carried out to detect any side effects.
他莫昔芬是一种被广泛用作乳腺癌化疗药物的药物。它需要长期服用，从几周至数年不等，因此可能引发妇科和非妇科并发症。
进行本研究以阐明服用他莫昔芬对大鼠卵巢卵泡的组织病理学影响，并评估停药后有望出现的恢复情况。
将24只成年雌性白化病大鼠随机分为四组。第一组：未接受治疗的对照大鼠。第二组：接受橄榄油载体的大鼠。第三组：大鼠通过口服给予溶解于橄榄油中的他莫昔芬，剂量为5毫克/千克，持续4周。第四组：大鼠按第三组方式接受他莫昔芬治疗，然后停药4周以观察恢复情况。随后，将大鼠麻醉并摘除卵巢，制备用于光学显微镜组织学评估的样本。
第三组大鼠卵巢的组织学检查结果显示闭锁卵泡增多、出现囊性卵巢卵泡和囊性黄体。卵巢卵泡的颗粒细胞排列紊乱，细胞质有空泡化，固缩核数量增加、核碎片化以及出现凋亡小体。停药后，卵巢组织有轻微改善，出现一些卵母细胞退化的闭锁卵泡和间质增生。
基于这些结果，他莫昔芬可引起卵巢明显的组织学变化。如果预防乳腺癌必须使用他莫昔芬，则应频繁进行妇科检查以检测任何副作用。
