Characterization of vacuolar membrane proteins changed in rice root treated with gibberellin.

作者信息

Konishi Hirosato, Maeshima Masayoshi, Komatsu Setsuko

机构信息

Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.

出版信息

J Proteome Res. 2005 Sep-Oct;4(5):1775-80. doi: 10.1021/pr050079c.

DOI:10.1021/pr050079c

PMID:16212432

Abstract

Rice vacuolar membrane proteins changed by gibberellin (GA) were analyzed using a proteome approach. Vacuolar membrane fractions were isolated using a discontinuous sucrose/sorbitol system and 10 proteins increased in vacuolar membrane of the root, treated with GA(3) as compared with control. Fructose-1,6-bisphosphate aldolase C-1 and vacuolar H(+)-ATPase (V-ATPase) increased in root vacuolar membrane by GA(3) interacted in rice roots. It suggests that aldolase C-1 regulates the V-ATPase mediated control of cell elongation that determines root growth.

摘要