Wuhrer Manfred, Deelder André M, Hokke Cornelis H
Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Oct 25;825(2):124-33. doi: 10.1016/j.jchromb.2005.01.030.
Liquid chromatography (LC)-mass spectrometry (MS) has developed into an invaluable technology for the analysis of protein glycosylation. This review focuses on the recent developments in LC and combinations thereof with MS for this field of research. Recently introduced methods for the structural analysis of released glycans (native or derivatised) as well as glycopeptides, on normal phase, reverse phase and graphitized carbon LC columns with online MS(/MS) will be reviewed. Performed on nano-scale or capillary-scale, these LC-MS methods operate at femtomole sensitivity and support the further integration of glycosylation analysis in proteomics methodology.
液相色谱（LC）-质谱（MS）已发展成为蛋白质糖基化分析中一项极具价值的技术。本综述聚焦于液相色谱及其与质谱联用技术在该研究领域的最新进展。本文将对近期采用正相、反相和石墨化碳液相色谱柱并结合在线质谱（/质谱）对释放的聚糖（天然或衍生化）以及糖肽进行结构分析的方法进行综述。这些液相色谱-质谱方法在纳升或毛细管规模上运行，具有飞摩尔灵敏度，支持糖基化分析在蛋白质组学方法中的进一步整合。
