从反刍动物乳腺炎分离出的金黄色葡萄球菌分泌的肠毒素和毒性休克综合征毒素-1的检测。酶联免疫吸附测定法（ELISA）与免疫印迹法的比较。

Detection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot.

作者信息

Orden J A, Goyache J, Hernández J, Doménech A, Suárez G, Gómez-Lucía E

机构信息

Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

出版信息

J Appl Bacteriol. 1992 Jun;72(6):486-9. doi: 10.1111/j.1365-2672.1992.tb01863.x.

DOI:10.1111/j.1365-2672.1992.tb01863.x

PMID:1644704

Abstract

The production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1) was studied in 81 strains of Staphylococcus aureus isolated from cases of mastitis in cattle, goats and sheep. SE and TSST-1 were detected by two techniques: ELISA double antibody sandwich, and an immunoblot technique combined with a semiautomated electrophoresis system. More Staph. aureus strains isolated from sheep produced enterotoxins than those from goats and cattle. SEC was the predominant type in all isolates from these animal species. The highest proportion of strains producing TSST-1 were obtained from sheep, twice as many as those from goats or cows. The two techniques gave similar results, as all the strains positive by immunoblot were also positive by ELISA, and only three were positive by ELISA but negative by immunoblot.

摘要

对从患乳腺炎的牛、山羊和绵羊病例中分离出的81株金黄色葡萄球菌进行了葡萄球菌肠毒素（SE）和中毒性休克综合征毒素-1（TSST-1）产生情况的研究。采用两种技术检测SE和TSST-1：ELISA双抗体夹心技术以及与半自动电泳系统相结合的免疫印迹技术。从绵羊分离出的金黄色葡萄球菌菌株产生肠毒素的比从山羊和牛分离出的更多。SEC是所有这些动物物种分离株中的主要类型。产生TSST-1的菌株比例最高的来自绵羊，是来自山羊或奶牛的两倍。这两种技术得出了相似的结果，因为所有免疫印迹呈阳性的菌株ELISA也呈阳性，只有三株ELISA呈阳性但免疫印迹呈阴性。