Eng-Wilmot D L, Martin D F
Microbios. 1979;26(104):103-13.
The assimilation of iron, a growth-limiting metal ion of the cytotoxic marine cyanobacterium, Gomphosphaeria aponina, has been examined in both static and steady-state cultures using 59Fe (III). Uptake of iron by cells followed first-order kinetics, and biphasic (absorption and uptake) behavior was observed as suggested by noted differences between cultures incubated in the light and in the dark. Iron removal in illuminated cultures was rapid, occurring at rates comparable to exponential growth rates. Although uptake was mediated by a chelating agent (EDTA), synthesis and iron assisted transport by hydroxamate-type siderophores was not involved in the uptake of iron by cells, as determined by standard chemical and biological assays of iron deficient cultures. The ecological implications of this research is considered with respect to the cytotoxic antagonism between the cyanobacterium and Florida's red tide organism, Pytochodiscus brevis (Gymnodinium breve).
使用59Fe（III）在静态和稳态培养中研究了铁（一种对细胞毒性海洋蓝细菌阿氏鞘丝藻生长有限制作用的金属离子）的同化作用。细胞对铁的摄取遵循一级动力学，并且观察到双相（吸收和摄取）行为，正如在光照和黑暗条件下培养的培养物之间的显著差异所表明的那样。光照培养物中铁的去除很快，其速率与指数生长速率相当。尽管摄取是由螯合剂（EDTA）介导的，但通过标准的化学和生物学测定法确定，细胞摄取铁的过程中不涉及异羟肟酸型铁载体的合成和铁辅助转运。关于蓝细菌与佛罗里达赤潮生物短裸甲藻（原称短裸藻）之间的细胞毒性拮抗作用，考虑了这项研究的生态学意义。
