与小鼠腭裂相关的一个新候选基因全长cDNA序列的克隆
Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate.
作者信息
Li Xin, Jin Yan, Yan Wei, Liu Yuan, Wang Xin-wen
机构信息
Department of Oral Pathology, Stomatology College, the Fourth Military Medical University, Xi'an, Shaanxi, 710032 PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Oct;22(5):481-4.
OBJECTIVE
Cloning and screening a novel candidate gene related to developing mouse cleft palate.
METHODS
The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases.
RESULTS
Four novel express sequence tags were obtained, one of which spanning 809 bp was the full-length of the novel gene cDNA identified by Northern hybridization.
CONCLUSION
A novel candidate gene related to mouse cleft palate was cloned.
目的
克隆并筛选与小鼠腭裂发生相关的新候选基因。
方法
采用改良的基于聚合酶链反应的消减杂交技术克隆差异表达基因。经反向斑点杂交鉴定后，对阳性克隆进行测序，并在GenBank数据库中分析其同源性。
结果
获得4个新的表达序列标签，其中1个长度为809 bp的序列经Northern杂交鉴定为新基因cDNA的全长。
结论
克隆了1个与小鼠腭裂相关的新候选基因。
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