褪黑素及其代谢产物的测量：对评估其生物学作用的重要性。
Measurement of melatonin and its metabolites: importance for the evaluation of their biological roles.
作者信息
Martinez Glaucia R, Almeida Eduardo A, Klitzke Clécio F, Onuki Janice, Prado Fernanda M, Medeiros Marisa H G, Di Mascio Paolo
机构信息
Departamento de Bioquímica e Biologia Molecular, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
出版信息
Endocrine. 2005 Jul;27(2):111-8. doi: 10.1385/endo:27:2:111.
Many physiologic changes related to light-dark cycles and antioxidant effects have been related to melatonin (N-acetyl-5-methoxytryptamine) and its metabolites, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and N1-acetyl-5-methoxykynuramine (AMK). In this review, we discuss some methodologies, in particular, those employing high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) assays to quantitatively determine melatonin, AFMK, and AMK. These approaches offer a highly specific and an accurate quantification of melatonin and its metabolites. These characteristics are essential to point out correctly the biological effects of these compounds in physiological and pathological conditions.
许多与昼夜节律和抗氧化作用相关的生理变化都与褪黑素（N-乙酰-5-甲氧基色胺）及其代谢产物N1-乙酰-N2-甲酰基-5-甲氧基犬尿胺（AFMK）和N1-乙酰-5-甲氧基犬尿胺（AMK）有关。在本综述中，我们讨论了一些方法，特别是那些采用高效液相色谱串联质谱（HPLC-MS/MS）分析法来定量测定褪黑素、AFMK和AMK的方法。这些方法能够对褪黑素及其代谢产物进行高度特异性和准确的定量。这些特性对于正确指出这些化合物在生理和病理条件下的生物学效应至关重要。
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