Modaressi Katy, Taylor K E, Bewtra J K, Biswas N
Department of Civil and Environmental Engineering, University of Windsor, Windsor, Ontario Canada N9B 3P4.
Water Res. 2005 Nov;39(18):4309-16. doi: 10.1016/j.watres.2005.08.005. Epub 2005 Oct 10.
The feasibility of using the enzyme laccase to treat synthetic wastewater containing bisphenol-A (BPA) was examined. Optimization of pH, laccase concentration, polyethylene glycol (PEG) as an additive for >95% conversion and precipitation of BPA over 3 h of reaction period was determined through colorimetric assay and HPLC. PEG reduced enzyme inactivation, allowing a 5.2-fold reduction in the amount of laccase required for >95% removal of BPA in the range of 0.1-1 mM over 3 h. The fate of PEG after the reaction was also monitored. Linear relationships were found between the concentration of BPA (0.1-1 mM) and the optimum concentrations of laccase and PEG. Little PEG remained in the solution when up to 75 mg/L of PEG was used to treat 0.5 mM BPA. Beyond this level, PEG concentration increased linearly in the supernatant. It is inferred that an interaction between PEG and the polymeric products resulted in the protection of laccase.
研究了使用漆酶处理含双酚A（BPA）的合成废水的可行性。通过比色法和高效液相色谱法确定了pH、漆酶浓度、作为添加剂的聚乙二醇（PEG）的优化条件，以实现>95%的转化率以及在3小时反应期内BPA的沉淀。PEG减少了酶的失活，使得在3小时内0.1 - 1 mM范围内>95%去除BPA所需的漆酶量减少了5.2倍。还监测了反应后PEG的去向。发现BPA浓度（0.1 - 1 mM）与漆酶和PEG的最佳浓度之间存在线性关系。当使用高达75 mg/L的PEG处理0.5 mM BPA时，溶液中残留的PEG很少。超过此水平，上清液中PEG浓度呈线性增加。据推测，PEG与聚合物产物之间的相互作用导致了漆酶的保护。
