Ordemann J, Höflich C, Braumann C, Hartmann J, Jacobi C A
Klinik für Allgemein-, Viszeral-, Gefäss- und Thoraxchirurgie, Medizinische Fakultät der Charité, Universitätsmedizin Berlin, Campus Mitte.
Zentralbl Chir. 2005 Oct;130(5):405-9. doi: 10.1055/s-2005-836837.
The mechanism of potential tumor cell spread and growth during laparoscopy is poorly understood. Nevertheless, different experimental studies reported a stimulation of tumor cell growth and an increased metastatic potential of carcinoma cells using carbon dioxide as an insufflation medium. Adhesion molecules do play an important and regulatory function in the process of metastatic spread and invasion of cancer cells. Therefore we investigated the influence of CO2 and Helium insufflation on the in-vitro expression of E-Cadherin, CD44v6 and CD54 (ICAM-1) on HT-29 colon carcinoma cells.
HT-29 carcinoma cells were exposed to either CO2 or helium insufflation. Expression of E-Cadherin, CD44v6 and CD54 (ICAM-1) on HT-29 colon carcinoma cells were measured 1, 12, 24, 48 and 96 h after CO2 and helium insufflation using flowcytometry (FACScan). Data were analyzed by Friedman-test.
HT-29 cell line showed a short decrease in E-Cadherin expression after CO2 exposure while helium insufflation had no influence. In contrasts to these findings the expression of CD44v6 and CD54 on HT-29 cells were not influenced significantly by either CO2 or helium.
CO2 seems only to have a minor influence on the expression of E-Cadherin while expression of other adhesion molecules did not change after CO2 incubation. The alternative gas helium did not cause any significant changes of the expression of either E-Cadherin, CD44v6 and CD54. Further investigations are needed to elucidate the changes of the metastatic potential of tumor cells after laparoscopic and open procedures.
腹腔镜检查过程中潜在肿瘤细胞扩散和生长的机制尚不清楚。然而，不同的实验研究报告称，使用二氧化碳作为充气介质会刺激肿瘤细胞生长并增加癌细胞的转移潜能。粘附分子在癌细胞转移扩散和侵袭过程中确实发挥着重要的调节作用。因此，我们研究了二氧化碳和氦气充气对HT-29结肠癌细胞中E-钙粘蛋白、CD44v6和CD54（细胞间粘附分子-1）体外表达的影响。
将HT-29癌细胞暴露于二氧化碳或氦气充气环境中。在二氧化碳和氦气充气后1、12、24、48和96小时，使用流式细胞仪（FACScan）测量HT-29结肠癌细胞上E-钙粘蛋白、CD44v6和CD54（细胞间粘附分子-1）的表达。数据采用Friedman检验进行分析。
HT-29细胞系在暴露于二氧化碳后E-钙粘蛋白表达短暂下降，而氦气充气则无影响。与这些发现相反，HT-29细胞上CD44v6和CD54的表达不受二氧化碳或氦气的显著影响。
二氧化碳似乎仅对E-钙粘蛋白的表达有轻微影响，而在二氧化碳孵育后其他粘附分子的表达没有变化。替代气体氦气并未导致E-钙粘蛋白、CD44v6和CD54的表达发生任何显著变化。需要进一步研究以阐明腹腔镜手术和开放手术后肿瘤细胞转移潜能的变化。
