Cai Wei, Dong Feng, Wang Zhengting, Yang Xiaohua, Zheng Minhua, Che Xiajing
Department of General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University , Shanghai .
Int J Hyperthermia. 2014 May;30(3):201-9. doi: 10.3109/02656736.2014.898339. Epub 2014 Apr 10.
Peritoneal carcinomatosis (PC) arising from colorectal cancer is associated with poor prognosis and few treatment options are currently available. Laparoscopic CO2 insufflation stimulates the progression and metastatic potential of gastrointestinal carcinomas. However, heated and humidified CO2 pneumoperitoneum (HH-CO2) is a promising treatment for PC, although its effects and mechanism of action in human colon cancer cells remain unclear. This study evaluated the anti-tumour effects of HH-CO2 on human colon cancer in vitro.
Cell viability was assessed using the WST-8 assay in two colon cancer cell lines. Apoptosis was assessed by Annexin V PI flow cytometry, and migration and invasion were examined using wound healing and Transwell® invasion assays. The expressions of Bcl-2, Bax, matrix metalloproteinase-2 (MMP-2), E-cadherin, ICAM-1, and CD44 were detected by western blotting.
HH-CO2 significantly inhibited cell proliferation, migration, invasion and adhesion. HH-CO2 induced apoptosis and significantly inhibited the expression of Bcl-2, MMP-2, ICAM-1 and CD44, and increased Bax and E-cadherin expression in colon cancer cells.
HH-CO2 induces apoptosis and inhibits proliferation, migration, invasion and adhesion of human colon cancer cells. Our results suggest that HH-CO2 may serve as a potential candidate for the treatment and/or prevention of peritoneal carcinomatosis from colorectal cancer and warrant further in vivo investigation.
结直肠癌引起的腹膜癌（PC）预后较差，目前可用的治疗选择较少。腹腔镜二氧化碳气腹会刺激胃肠道癌的进展和转移潜能。然而，温热加湿二氧化碳气腹（HH-CO2）对PC是一种有前景的治疗方法，尽管其在人结肠癌细胞中的作用效果和作用机制尚不清楚。本研究在体外评估了HH-CO2对人结肠癌的抗肿瘤作用。
使用WST-8法评估两种结肠癌细胞系的细胞活力。通过膜联蛋白V/PI流式细胞术评估细胞凋亡，使用伤口愈合实验和Transwell®侵袭实验检测细胞迁移和侵袭能力。通过蛋白质印迹法检测Bcl-2、Bax、基质金属蛋白酶-2（MMP-2）、E-钙黏蛋白、细胞间黏附分子-1（ICAM-1）和CD44的表达。
HH-CO2显著抑制细胞增殖、迁移、侵袭和黏附。HH-CO2诱导细胞凋亡，并显著抑制结肠癌细胞中Bcl-2、MMP-2、ICAM-1和CD44的表达，增加Bax和E-钙黏蛋白的表达。
HH-CO2诱导细胞凋亡，抑制人结肠癌细胞的增殖、迁移、侵袭和黏附。我们的结果表明，HH-CO2可能作为治疗和/或预防结直肠癌腹膜癌的潜在候选方法，值得进一步进行体内研究。
