一种用于检测养殖虹鳟鱼中传染性胰腺坏死病毒（IPNV）的可靠逆转录聚合酶链反应-酶联免疫吸附测定方法。
A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout.
作者信息
Milne S A, Gallacher S, Cash P, Porter A J R
机构信息
Department of Molecular and Cell Biology, School of Medical Sciences, University of Aberdeen, I.M.S Building, Foresterhill, Aberdeen AB25 2ZD, Scotland.
出版信息
J Virol Methods. 2006 Mar;132(1-2):92-6. doi: 10.1016/j.jviromet.2005.09.003. Epub 2005 Oct 10.
A new method, termed RT-PCR-ELISA, was evaluated for ease of use, reliability and sensitivity when detecting infectious pancreatic necrosis virus (IPNV) present in trout kidney tissue. The method had comparable sensitivity to existing PCR assays and could successfully detect 1.5 x 10(4) pfu IPNV in artificially contaminated trout kidney samples. The technique was easily established in a new laboratory and required no specialised equipment. The method had a high sample throughput capable of screening 96 samples per run, making the technique extremely time efficient. The RT-PCR-ELISA is a safe, quick, reliable technique, which has the potential for use as a standard virus detection method.
一种名为RT-PCR-ELISA的新方法，在检测鲑鱼肾脏组织中存在的传染性胰腺坏死病毒（IPNV）时，对其易用性、可靠性和灵敏度进行了评估。该方法与现有的PCR检测方法具有相当的灵敏度，能够在人工污染的鲑鱼肾脏样本中成功检测到1.5×10⁴ 噬斑形成单位（pfu）的IPNV。该技术在新实验室中易于建立，且不需要专门的设备。该方法具有较高的样本通量，每次运行能够筛选96个样本，使得该技术极具时间效率。RT-PCR-ELISA是一种安全、快速、可靠的技术，有潜力用作标准的病毒检测方法。
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